Construct: shRNA TRCN0000129286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024085.2-1508s1c1
DNA Barcode:
CTTCCAGTACAAGGCAGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATG9A (79065)
Gene Description:
autophagy related 9A
Transcript:
RefSeq NM_024085.2 (NON-CURRENT)
Match location:
Position 1508 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCAGTACAAGGCAGTGTT
Hairpin Sequence:
5'-CCGG-CTTCCAGTACAAGGCAGTGTT-CTCGAG-AACACTGCCTTGTACTGGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCAGTACAAGGCAGTGTTCTCGAGAACACTGCCTTGTACTGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCAGTACAAGGCAGTGTTCTCGAGAACACTGCCTTGTACTGGAAG-3'

Other clones with same target sequence:

(none)