Construct: shRNA TRCN0000129338

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032127.1-1180s1c1
DNA Barcode:
CCCTTGCACTCTTCATGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM160A2 (84067)
Gene Description:
family with sequence similarity 160 member A2
Transcript:
RefSeq NM_032127.1 (NON-CURRENT)
Match location:
Position 1180 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129338)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGCACTCTTCATGAGTT
Hairpin Sequence:
5'-CCGG-CCCTTGCACTCTTCATGAGTT-CTCGAG-AACTCATGAAGAGTGCAAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGCACTCTTCATGAGTTCTCGAGAACTCATGAAGAGTGCAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGCACTCTTCATGAGTTCTCGAGAACTCATGAAGAGTGCAAGGG-3'

Other clones with same target sequence:

(none)