Construct: shRNA TRCN0000129372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138408.1-352s1c1
DNA Barcode:
GCTCAGCATGACAAGAACTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF3C6 (112495)
Gene Description:
general transcription factor IIIC subunit 6
Transcript:
RefSeq NM_138408.1 (NON-CURRENT)
Match location:
Position 352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGCATGACAAGAACTCT
Hairpin Sequence:
5'-CCGG-GCTCAGCATGACAAGAACTCT-CTCGAG-AGAGTTCTTGTCATGCTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGCATGACAAGAACTCTCTCGAGAGAGTTCTTGTCATGCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGCATGACAAGAACTCTCTCGAGAGAGTTCTTGTCATGCTGAGC-3'

Other clones with same target sequence:

(none)