Construct: shRNA TRCN0000129380

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015116.1-1924s1c1
DNA Barcode:
GCAGTTTACAATCCGGAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRCH1 (23143)
Gene Description:
leucine rich repeats and calponin homology domain containing 1
Transcript:
RefSeq NM_015116.1 (NON-CURRENT)
Match location:
Position 1924 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129380)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTTACAATCCGGAGGAA
Hairpin Sequence:
5'-CCGG-GCAGTTTACAATCCGGAGGAA-CTCGAG-TTCCTCCGGATTGTAAACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTTACAATCCGGAGGAACTCGAGTTCCTCCGGATTGTAAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTTACAATCCGGAGGAACTCGAGTTCCTCCGGATTGTAAACTGC-3'

Other clones with same target sequence:

(none)