Construct: shRNA TRCN0000129432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181719.2-1462s1c1
DNA Barcode:
GCCAGAGTCATCTACTTCTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMCO4 (255104)
Gene Description:
transmembrane and coiled-coil domains 4
Transcript:
RefSeq NM_181719.2 (NON-CURRENT)
Match location:
Position 1462 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAGTCATCTACTTCTGT
Hairpin Sequence:
5'-CCGG-GCCAGAGTCATCTACTTCTGT-CTCGAG-ACAGAAGTAGATGACTCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAGTCATCTACTTCTGTCTCGAGACAGAAGTAGATGACTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAGTCATCTACTTCTGTCTCGAGACAGAAGTAGATGACTCTGGC-3'

Other clones with same target sequence:

(none)