Construct: shRNA TRCN0000129437

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018142.1-2231s1c1
DNA Barcode:
GAGAAGACACTCAGCAGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INTS10 (55174)
Gene Description:
integrator complex subunit 10
Transcript:
RefSeq NM_018142.1 (NON-CURRENT)
Match location:
Position 2231 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129437)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGACACTCAGCAGTGAT
Hairpin Sequence:
5'-CCGG-GAGAAGACACTCAGCAGTGAT-CTCGAG-ATCACTGCTGAGTGTCTTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGACACTCAGCAGTGATCTCGAGATCACTGCTGAGTGTCTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGACACTCAGCAGTGATCTCGAGATCACTGCTGAGTGTCTTCTC-3'

Other clones with same target sequence:

(none)