Construct: shRNA TRCN0000129468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198485.1-279s1c1
DNA Barcode:
GCCAAGACAGATTTCAAGGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPRG1 (285386)
Gene Description:
tumor protein p63 regulated 1
Transcript:
RefSeq NM_198485.1 (NON-CURRENT)
Match location:
Position 279 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGACAGATTTCAAGGCA
Hairpin Sequence:
5'-CCGG-GCCAAGACAGATTTCAAGGCA-CTCGAG-TGCCTTGAAATCTGTCTTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGACAGATTTCAAGGCACTCGAGTGCCTTGAAATCTGTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGACAGATTTCAAGGCACTCGAGTGCCTTGAAATCTGTCTTGGC-3'

Other clones with same target sequence:

(none)