Construct: shRNA TRCN0000129514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015116.1-1398s1c1
DNA Barcode:
GCACCAACTCAGGAGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRCH1 (23143)
Gene Description:
leucine rich repeats and calponin homology domain containing 1
Transcript:
RefSeq NM_015116.1 (NON-CURRENT)
Match location:
Position 1398 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCAACTCAGGAGAAGAAA
Hairpin Sequence:
5'-CCGG-GCACCAACTCAGGAGAAGAAA-CTCGAG-TTTCTTCTCCTGAGTTGGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCAACTCAGGAGAAGAAACTCGAGTTTCTTCTCCTGAGTTGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCAACTCAGGAGAAGAAACTCGAGTTTCTTCTCCTGAGTTGGTGC-3'

Other clones with same target sequence:

(none)