Construct: shRNA TRCN0000129521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020863.2-1219s1c1
DNA Barcode:
GCACAAGAAGATCAAGCAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFAT (57623)
Gene Description:
zinc finger and AT-hook domain containing
Transcript:
RefSeq NM_020863.2 (NON-CURRENT)
Match location:
Position 1219 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAGAAGATCAAGCAGCA
Hairpin Sequence:
5'-CCGG-GCACAAGAAGATCAAGCAGCA-CTCGAG-TGCTGCTTGATCTTCTTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAGAAGATCAAGCAGCACTCGAGTGCTGCTTGATCTTCTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAGAAGATCAAGCAGCACTCGAGTGCTGCTTGATCTTCTTGTGC-3'

Other clones with same target sequence:

(none)