Construct: shRNA TRCN0000129545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020909.2-1493s1c1
DNA Barcode:
GCCCAGACTTATTGGAAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPB41L5 (57669)
Gene Description:
erythrocyte membrane protein band 4.1 like 5
Transcript:
RefSeq NM_020909.2 (NON-CURRENT)
Match location:
Position 1493 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGACTTATTGGAAGCAA
Hairpin Sequence:
5'-CCGG-GCCCAGACTTATTGGAAGCAA-CTCGAG-TTGCTTCCAATAAGTCTGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGACTTATTGGAAGCAACTCGAGTTGCTTCCAATAAGTCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGACTTATTGGAAGCAACTCGAGTTGCTTCCAATAAGTCTGGGC-3'

Other clones with same target sequence:

(none)