Construct: shRNA TRCN0000129574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020940.2-3609s1c1
DNA Barcode:
CCGCAGCACTTTCTTCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM160B1 (57700)
Gene Description:
family with sequence similarity 160 member B1
Transcript:
RefSeq NM_020940.2 (NON-CURRENT)
Match location:
Position 3609 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAGCACTTTCTTCTTCAT
Hairpin Sequence:
5'-CCGG-CCGCAGCACTTTCTTCTTCAT-CTCGAG-ATGAAGAAGAAAGTGCTGCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAGCACTTTCTTCTTCATCTCGAGATGAAGAAGAAAGTGCTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAGCACTTTCTTCTTCATCTCGAGATGAAGAAGAAAGTGCTGCGG-3'

Other clones with same target sequence:

(none)