Construct: shRNA TRCN0000129604

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031471.4-976s1c1
DNA Barcode:
CCTGCAGTACCACATCAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FERMT3 (83706)
Gene Description:
fermitin family member 3
Transcript:
RefSeq NM_031471.4 (NON-CURRENT)
Match location:
Position 976 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129604)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAGTACCACATCAACAA
Hairpin Sequence:
5'-CCGG-CCTGCAGTACCACATCAACAA-CTCGAG-TTGTTGATGTGGTACTGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAGTACCACATCAACAACTCGAGTTGTTGATGTGGTACTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAGTACCACATCAACAACTCGAGTTGTTGATGTGGTACTGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000438252