Construct: shRNA TRCN0000129621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033415.2-654s1c1
DNA Barcode:
CATCATCTTCACTGCCTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARMC6 (93436)
Gene Description:
armadillo repeat containing 6
Transcript:
RefSeq NM_033415.2 (NON-CURRENT)
Match location:
Position 654 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCATCTTCACTGCCTGGAA
Hairpin Sequence:
5'-CCGG-CATCATCTTCACTGCCTGGAA-CTCGAG-TTCCAGGCAGTGAAGATGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCATCTTCACTGCCTGGAACTCGAGTTCCAGGCAGTGAAGATGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCATCTTCACTGCCTGGAACTCGAGTTCCAGGCAGTGAAGATGATG-3'

Other clones with same target sequence:

(none)