Construct: shRNA TRCN0000129750
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005362.3-1401s1c1
- DNA Barcode:
- CCAGGTTTATGAATGACAGTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAGEA3 (4102)
- Gene Description:
- MAGE family member A3
- Transcript:
- RefSeq NM_005362.3 (NON-CURRENT)
- Match location:
- Position 1401 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000129750)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGGTTTATGAATGACAGTA
- Hairpin Sequence:
-
5'-CCGG-CCAGGTTTATGAATGACAGTA-CTCGAG-TACTGTCATTCATAAACCTGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGGTTTATGAATGACAGTACTCGAGTACTGTCATTCATAAACCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGGTTTATGAATGACAGTACTCGAGTACTGTCATTCATAAACCTGG-3'
Other clones with same target sequence:
(none)
