Construct: shRNA TRCN0000129851

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177452.2-462s1c1
DNA Barcode:
CGACAATCTAAGGACAAATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAPPC6B (122553)
Gene Description:
trafficking protein particle complex 6B
Transcript:
RefSeq NM_177452.2 (NON-CURRENT)
Match location:
Position 462 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129851)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACAATCTAAGGACAAATCA
Hairpin Sequence:
5'-CCGG-CGACAATCTAAGGACAAATCA-CTCGAG-TGATTTGTCCTTAGATTGTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACAATCTAAGGACAAATCACTCGAGTGATTTGTCCTTAGATTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACAATCTAAGGACAAATCACTCGAGTGATTTGTCCTTAGATTGTCG-3'

Other clones with same target sequence:

(none)