Construct: shRNA TRCN0000129937

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_374435.2-874s1c1
DNA Barcode:
GCAATGGTTATAAGGATGAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C7orf57 (136288)
Gene Description:
chromosome 7 open reading frame 57
Transcript:
RefSeq XM_374435.2 (NON-CURRENT)
Match location:
Position 874 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129937)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGGTTATAAGGATGAGT
Hairpin Sequence:
5'-CCGG-GCAATGGTTATAAGGATGAGT-CTCGAG-ACTCATCCTTATAACCATTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGGTTATAAGGATGAGTCTCGAGACTCATCCTTATAACCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGGTTATAAGGATGAGTCTCGAGACTCATCCTTATAACCATTGC-3'

Other clones with same target sequence:

(none)