Construct: shRNA TRCN0000129939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005768.5-1505s1c1
DNA Barcode:
GCCTACTATTCATATTGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LPCAT3 (10162)
Gene Description:
lysophosphatidylcholine acyltransferase 3
Transcript:
RefSeq NM_005768.5 (NON-CURRENT)
Match location:
Position 1505 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACTATTCATATTGCCTT
Hairpin Sequence:
5'-CCGG-GCCTACTATTCATATTGCCTT-CTCGAG-AAGGCAATATGAATAGTAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACTATTCATATTGCCTTCTCGAGAAGGCAATATGAATAGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACTATTCATATTGCCTTCTCGAGAAGGCAATATGAATAGTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000121439
  2. TRCN0000280675