Construct: shRNA TRCN0000129997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014398.2-388s1c1
DNA Barcode:
GATGGTCATATCACCTTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LAMP3 (27074)
Gene Description:
lysosomal associated membrane protein 3
Transcript:
RefSeq NM_014398.2 (NON-CURRENT)
Match location:
Position 388 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000129997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGTCATATCACCTTTCAA
Hairpin Sequence:
5'-CCGG-GATGGTCATATCACCTTTCAA-CTCGAG-TTGAAAGGTGATATGACCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGTCATATCACCTTTCAACTCGAGTTGAAAGGTGATATGACCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGTCATATCACCTTTCAACTCGAGTTGAAAGGTGATATGACCATC-3'

Other clones with same target sequence:

(none)