Construct: shRNA TRCN0000130051

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153603.1-1965s1c1
DNA Barcode:
GCTGTTGCTTATTTCGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COG7 (91949)
Gene Description:
component of oligomeric golgi complex 7
Transcript:
RefSeq NM_153603.1 (NON-CURRENT)
Match location:
Position 1965 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130051)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGCTTATTTCGAAGAT
Hairpin Sequence:
5'-CCGG-GCTGTTGCTTATTTCGAAGAT-CTCGAG-ATCTTCGAAATAAGCAACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGCTTATTTCGAAGATCTCGAGATCTTCGAAATAAGCAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGCTTATTTCGAAGATCTCGAGATCTTCGAAATAAGCAACAGC-3'

Other clones with same target sequence:

(none)