Construct: shRNA TRCN0000130180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018325.1-2004s1c1
DNA Barcode:
CCACAGAAAGAAAGTGAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C9orf72 (203228)
Gene Description:
C9orf72-SMCR8 complex subunit
Transcript:
RefSeq NM_018325.1 (NON-CURRENT)
Match location:
Position 2004 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGAAAGAAAGTGAGCTT
Hairpin Sequence:
5'-CCGG-CCACAGAAAGAAAGTGAGCTT-CTCGAG-AAGCTCACTTTCTTTCTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGAAAGAAAGTGAGCTTCTCGAGAAGCTCACTTTCTTTCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGAAAGAAAGTGAGCTTCTCGAGAAGCTCACTTTCTTTCTGTGG-3'

Other clones with same target sequence:

(none)