Construct: shRNA TRCN0000130196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173812.3-1568s1c1
DNA Barcode:
CGCTGAGATACACAAAGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPY19L2 (283417)
Gene Description:
dpy-19 like 2
Transcript:
RefSeq NM_173812.3 (NON-CURRENT)
Match location:
Position 1568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGAGATACACAAAGACAT
Hairpin Sequence:
5'-CCGG-CGCTGAGATACACAAAGACAT-CTCGAG-ATGTCTTTGTGTATCTCAGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGAGATACACAAAGACATCTCGAGATGTCTTTGTGTATCTCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGAGATACACAAAGACATCTCGAGATGTCTTTGTGTATCTCAGCG-3'

Other clones with same target sequence:

(none)