Construct: shRNA TRCN0000130213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_375355.1-2239s1c1
DNA Barcode:
CGTGATCCTAATGTCACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
N4BP1 (9683)
Gene Description:
NEDD4 binding protein 1
Transcript:
RefSeq XM_375355.1 (NON-CURRENT)
Match location:
Position 2239 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGATCCTAATGTCACAGAA
Hairpin Sequence:
5'-CCGG-CGTGATCCTAATGTCACAGAA-CTCGAG-TTCTGTGACATTAGGATCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGATCCTAATGTCACAGAACTCGAGTTCTGTGACATTAGGATCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGATCCTAATGTCACAGAACTCGAGTTCTGTGACATTAGGATCACG-3'

Other clones with same target sequence:

  1. TRCN0000277610