Construct: shRNA TRCN0000130271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052970.3-2142s1c1
DNA Barcode:
CCATCGACTTTCTTTCCAACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSPA12B (116835)
Gene Description:
heat shock protein family A (Hsp70) member 12B
Transcript:
RefSeq NM_052970.3 (NON-CURRENT)
Match location:
Position 2142 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCGACTTTCTTTCCAACT
Hairpin Sequence:
5'-CCGG-CCATCGACTTTCTTTCCAACT-CTCGAG-AGTTGGAAAGAAAGTCGATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCGACTTTCTTTCCAACTCTCGAGAGTTGGAAAGAAAGTCGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCGACTTTCTTTCCAACTCTCGAGAGTTGGAAAGAAAGTCGATGG-3'

Other clones with same target sequence:

(none)