Construct: shRNA TRCN0000130316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207514.1-3380s1c1
DNA Barcode:
GCCATTCCGAAATCTGTGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DEF8 (54849)
Gene Description:
differentially expressed in FDCP 8 homolog
Transcript:
RefSeq NM_207514.1 (NON-CURRENT)
Match location:
Position 3380 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTCCGAAATCTGTGTAA
Hairpin Sequence:
5'-CCGG-GCCATTCCGAAATCTGTGTAA-CTCGAG-TTACACAGATTTCGGAATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTCCGAAATCTGTGTAACTCGAGTTACACAGATTTCGGAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTCCGAAATCTGTGTAACTCGAGTTACACAGATTTCGGAATGGC-3'

Other clones with same target sequence:

(none)