Construct: shRNA TRCN0000130336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018569.2-2114s1c1
DNA Barcode:
GCCTTCCTTTACCTTGTAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP1AR (55435)
Gene Description:
adaptor related protein complex 1 associated regulatory protein
Transcript:
RefSeq NM_018569.2 (NON-CURRENT)
Match location:
Position 2114 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCCTTTACCTTGTAGTA
Hairpin Sequence:
5'-CCGG-GCCTTCCTTTACCTTGTAGTA-CTCGAG-TACTACAAGGTAAAGGAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCCTTTACCTTGTAGTACTCGAGTACTACAAGGTAAAGGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCCTTTACCTTGTAGTACTCGAGTACTACAAGGTAAAGGAAGGC-3'

Other clones with same target sequence:

(none)