Construct: shRNA TRCN0000130369

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133372.1-214s1c1
DNA Barcode:
GCCAGATTCGACTGATTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FNIP1 (96459)
Gene Description:
folliculin interacting protein 1
Transcript:
RefSeq NM_133372.1 (NON-CURRENT)
Match location:
Position 214 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130369)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGATTCGACTGATTGTAT
Hairpin Sequence:
5'-CCGG-GCCAGATTCGACTGATTGTAT-CTCGAG-ATACAATCAGTCGAATCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGATTCGACTGATTGTATCTCGAGATACAATCAGTCGAATCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGATTCGACTGATTGTATCTCGAGATACAATCAGTCGAATCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000047146