Construct: shRNA TRCN0000130375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021824.1-721s1c1
DNA Barcode:
GTGAAAGGAATTGACGGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NIF3L1 (60491)
Gene Description:
NGG1 interacting factor 3 like 1
Transcript:
RefSeq NM_021824.1 (NON-CURRENT)
Match location:
Position 721 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAAGGAATTGACGGTGTT
Hairpin Sequence:
5'-CCGG-GTGAAAGGAATTGACGGTGTT-CTCGAG-AACACCGTCAATTCCTTTCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAAGGAATTGACGGTGTTCTCGAGAACACCGTCAATTCCTTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAAGGAATTGACGGTGTTCTCGAGAACACCGTCAATTCCTTTCAC-3'

Other clones with same target sequence:

(none)