Construct: shRNA TRCN0000130380
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_030571.2-831s1c1
- DNA Barcode:
- GCCAGAAACTTTCTCAAATCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NDFIP1 (80762)
- Gene Description:
- Nedd4 family interacting protein 1
- Transcript:
- RefSeq NM_030571.2 (NON-CURRENT)
- Match location:
- Position 831 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000130380)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCAGAAACTTTCTCAAATCT
- Hairpin Sequence:
-
5'-CCGG-GCCAGAAACTTTCTCAAATCT-CTCGAG-AGATTTGAGAAAGTTTCTGGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCAGAAACTTTCTCAAATCTCTCGAGAGATTTGAGAAAGTTTCTGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCAGAAACTTTCTCAAATCTCTCGAGAGATTTGAGAAAGTTTCTGGC-3'
Other clones with same target sequence:
-
TRCN0000281984
