Construct: shRNA TRCN0000130404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024101.4-1180s1c1
DNA Barcode:
GTCTTCTGAGAGTCAGATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MLPH (79083)
Gene Description:
melanophilin
Transcript:
RefSeq NM_024101.4 (NON-CURRENT)
Match location:
Position 1180 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTCTGAGAGTCAGATCTT
Hairpin Sequence:
5'-CCGG-GTCTTCTGAGAGTCAGATCTT-CTCGAG-AAGATCTGACTCTCAGAAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTCTGAGAGTCAGATCTTCTCGAGAAGATCTGACTCTCAGAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTCTGAGAGTCAGATCTTCTCGAGAAGATCTGACTCTCAGAAGAC-3'

Other clones with same target sequence:

  1. TRCN0000319072