Construct: shRNA TRCN0000130476

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182564.1-1375s1c1
DNA Barcode:
GCCTTCTTCATGCATGTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LINC00469 (283982)
Gene Description:
long intergenic non-protein coding RNA 469
Transcript:
RefSeq NM_182564.1 (NON-CURRENT)
Match location:
Position 1375 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130476)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTTCATGCATGTGATT
Hairpin Sequence:
5'-CCGG-GCCTTCTTCATGCATGTGATT-CTCGAG-AATCACATGCATGAAGAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTTCATGCATGTGATTCTCGAGAATCACATGCATGAAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTTCATGCATGTGATTCTCGAGAATCACATGCATGAAGAAGGC-3'

Other clones with same target sequence:

(none)