Construct: shRNA TRCN0000130515

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032325.2-525s1c1
DNA Barcode:
GAAGGCTGAAATCTCGTTTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF1AD (84285)
Gene Description:
eukaryotic translation initiation factor 1A domain containing
Transcript:
RefSeq NM_032325.2 (NON-CURRENT)
Match location:
Position 525 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130515)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGCTGAAATCTCGTTTGT
Hairpin Sequence:
5'-CCGG-GAAGGCTGAAATCTCGTTTGT-CTCGAG-ACAAACGAGATTTCAGCCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGCTGAAATCTCGTTTGTCTCGAGACAAACGAGATTTCAGCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGCTGAAATCTCGTTTGTCTCGAGACAAACGAGATTTCAGCCTTC-3'

Other clones with same target sequence:

(none)