Construct: shRNA TRCN0000130535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201280.1-441s1c1
DNA Barcode:
GTAGCTAGTGAGAAACAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BLOC1S5 (63915)
Gene Description:
biogenesis of lysosomal organelles complex 1 subunit 5
Transcript:
RefSeq NM_201280.1 (NON-CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGCTAGTGAGAAACAGCAT
Hairpin Sequence:
5'-CCGG-GTAGCTAGTGAGAAACAGCAT-CTCGAG-ATGCTGTTTCTCACTAGCTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGCTAGTGAGAAACAGCATCTCGAGATGCTGTTTCTCACTAGCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGCTAGTGAGAAACAGCATCTCGAGATGCTGTTTCTCACTAGCTAC-3'

Other clones with same target sequence:

(none)