Construct: shRNA TRCN0000130539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022918.2-2925s1c1
DNA Barcode:
GCTTTGTAGAACTGACCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM135 (65084)
Gene Description:
transmembrane protein 135
Transcript:
RefSeq NM_022918.2 (NON-CURRENT)
Match location:
Position 2925 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGTAGAACTGACCTGTT
Hairpin Sequence:
5'-CCGG-GCTTTGTAGAACTGACCTGTT-CTCGAG-AACAGGTCAGTTCTACAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGTAGAACTGACCTGTTCTCGAGAACAGGTCAGTTCTACAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGTAGAACTGACCTGTTCTCGAGAACAGGTCAGTTCTACAAAGC-3'

Other clones with same target sequence:

(none)