Construct: shRNA TRCN0000130598

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024545.2-2132s1c1
DNA Barcode:
CCAAGCATACTCCGGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAP130 (79595)
Gene Description:
Sin3A associated protein 130
Transcript:
RefSeq NM_024545.2 (NON-CURRENT)
Match location:
Position 2132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130598)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCATACTCCGGAAGAAA
Hairpin Sequence:
5'-CCGG-CCAAGCATACTCCGGAAGAAA-CTCGAG-TTTCTTCCGGAGTATGCTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCATACTCCGGAAGAAACTCGAGTTTCTTCCGGAGTATGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCATACTCCGGAAGAAACTCGAGTTTCTTCCGGAGTATGCTTGG-3'

Other clones with same target sequence:

  1. TRCN0000352903