Construct: shRNA TRCN0000130626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014858.2-2157s1c1
DNA Barcode:
CCTGACTGAGCTTCATCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMCC2 (9911)
Gene Description:
transmembrane and coiled-coil domain family 2
Transcript:
RefSeq NM_014858.2 (NON-CURRENT)
Match location:
Position 2157 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACTGAGCTTCATCAGAA
Hairpin Sequence:
5'-CCGG-CCTGACTGAGCTTCATCAGAA-CTCGAG-TTCTGATGAAGCTCAGTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACTGAGCTTCATCAGAACTCGAGTTCTGATGAAGCTCAGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACTGAGCTTCATCAGAACTCGAGTTCTGATGAAGCTCAGTCAGG-3'

Other clones with same target sequence:

(none)