Construct: shRNA TRCN0000130668

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371459.2-2426s1c1
DNA Barcode:
CCATTGCTTTGGAGCACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUCD1 (83606)
Gene Description:
guanylyl cyclase domain containing 1
Transcript:
RefSeq XM_371459.2 (NON-CURRENT)
Match location:
Position 2426 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130668)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGCTTTGGAGCACAGAT
Hairpin Sequence:
5'-CCGG-CCATTGCTTTGGAGCACAGAT-CTCGAG-ATCTGTGCTCCAAAGCAATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGCTTTGGAGCACAGATCTCGAGATCTGTGCTCCAAAGCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGCTTTGGAGCACAGATCTCGAGATCTGTGCTCCAAAGCAATGG-3'

Other clones with same target sequence:

(none)