Construct: shRNA TRCN0000130726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015245.1-3455s1c1
DNA Barcode:
GATATGGACCAAGATGCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKS1A (23294)
Gene Description:
ankyrin repeat and sterile alpha motif domain containing 1A
Transcript:
RefSeq NM_015245.1 (NON-CURRENT)
Match location:
Position 3455 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATATGGACCAAGATGCCCAA
Hairpin Sequence:
5'-CCGG-GATATGGACCAAGATGCCCAA-CTCGAG-TTGGGCATCTTGGTCCATATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATATGGACCAAGATGCCCAACTCGAGTTGGGCATCTTGGTCCATATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATATGGACCAAGATGCCCAACTCGAGTTGGGCATCTTGGTCCATATC-3'

Other clones with same target sequence:

(none)