Construct: shRNA TRCN0000130728

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018261.2-3152s1c1
DNA Barcode:
GCATTTGGAGTTCTTCAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXOC1 (55763)
Gene Description:
exocyst complex component 1
Transcript:
RefSeq NM_018261.2 (NON-CURRENT)
Match location:
Position 3152 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130728)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTTGGAGTTCTTCAGCTT
Hairpin Sequence:
5'-CCGG-GCATTTGGAGTTCTTCAGCTT-CTCGAG-AAGCTGAAGAACTCCAAATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTTGGAGTTCTTCAGCTTCTCGAGAAGCTGAAGAACTCCAAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTTGGAGTTCTTCAGCTTCTCGAGAAGCTGAAGAACTCCAAATGC-3'

Other clones with same target sequence:

(none)