Construct: shRNA TRCN0000130738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018465.1-265s1c1
DNA Barcode:
GCTTATGAATGCTCGACTTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLGRKT (55848)
Gene Description:
plasminogen receptor with a C-terminal lysine
Transcript:
RefSeq NM_018465.1 (NON-CURRENT)
Match location:
Position 265 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTATGAATGCTCGACTTCA
Hairpin Sequence:
5'-CCGG-GCTTATGAATGCTCGACTTCA-CTCGAG-TGAAGTCGAGCATTCATAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTATGAATGCTCGACTTCACTCGAGTGAAGTCGAGCATTCATAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTATGAATGCTCGACTTCACTCGAGTGAAGTCGAGCATTCATAAGC-3'

Other clones with same target sequence:

  1. TRCN0000352746