Construct: shRNA TRCN0000130749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018684.1-299s1c1
DNA Barcode:
GCTGACATCAATGTGATGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZC4H2 (55906)
Gene Description:
zinc finger C4H2-type containing
Transcript:
RefSeq NM_018684.1 (NON-CURRENT)
Match location:
Position 299 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACATCAATGTGATGGAA
Hairpin Sequence:
5'-CCGG-GCTGACATCAATGTGATGGAA-CTCGAG-TTCCATCACATTGATGTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACATCAATGTGATGGAACTCGAGTTCCATCACATTGATGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACATCAATGTGATGGAACTCGAGTTCCATCACATTGATGTCAGC-3'

Other clones with same target sequence:

(none)