Construct: shRNA TRCN0000130811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153756.1-286s1c1
DNA Barcode:
GAGGAGGATACGGAGTACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FNDC5 (252995)
Gene Description:
fibronectin type III domain containing 5
Transcript:
RefSeq NM_153756.1 (NON-CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGGATACGGAGTACATA
Hairpin Sequence:
5'-CCGG-GAGGAGGATACGGAGTACATA-CTCGAG-TATGTACTCCGTATCCTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGGATACGGAGTACATACTCGAGTATGTACTCCGTATCCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGGATACGGAGTACATACTCGAGTATGTACTCCGTATCCTCCTC-3'

Other clones with same target sequence:

(none)