Construct: shRNA TRCN0000130893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033446.1-650s1c1
DNA Barcode:
GAATGGGCAGAGTACCAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MVB12B (89853)
Gene Description:
multivesicular body subunit 12B
Transcript:
RefSeq NM_033446.1 (NON-CURRENT)
Match location:
Position 650 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGGGCAGAGTACCAAGAA
Hairpin Sequence:
5'-CCGG-GAATGGGCAGAGTACCAAGAA-CTCGAG-TTCTTGGTACTCTGCCCATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGGGCAGAGTACCAAGAACTCGAGTTCTTGGTACTCTGCCCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGGGCAGAGTACCAAGAACTCGAGTTCTTGGTACTCTGCCCATTC-3'

Other clones with same target sequence:

(none)