Construct: shRNA TRCN0000130907

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153013.2-473s1c1
DNA Barcode:
GCTAAGCTTGAATCAGCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NADK2 (133686)
Gene Description:
NAD kinase 2, mitochondrial
Transcript:
RefSeq NM_153013.2 (NON-CURRENT)
Match location:
Position 473 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130907)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAGCTTGAATCAGCACAA
Hairpin Sequence:
5'-CCGG-GCTAAGCTTGAATCAGCACAA-CTCGAG-TTGTGCTGATTCAAGCTTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAGCTTGAATCAGCACAACTCGAGTTGTGCTGATTCAAGCTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAGCTTGAATCAGCACAACTCGAGTTGTGCTGATTCAAGCTTAGC-3'

Other clones with same target sequence:

(none)