Construct: shRNA TRCN0000130915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130768.1-546s1c1
DNA Barcode:
GCAGAAGTTAATACCCAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASZ1 (136991)
Gene Description:
ankyrin repeat, SAM and basic leucine zipper domain containing 1
Transcript:
RefSeq NM_130768.1 (NON-CURRENT)
Match location:
Position 546 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000130915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGTTAATACCCAGGAT
Hairpin Sequence:
5'-CCGG-GCAGAAGTTAATACCCAGGAT-CTCGAG-ATCCTGGGTATTAACTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGTTAATACCCAGGATCTCGAGATCCTGGGTATTAACTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGTTAATACCCAGGATCTCGAGATCCTGGGTATTAACTTCTGC-3'

Other clones with same target sequence:

(none)