Construct: shRNA TRCN0000131012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198565.1-1679s1c1
DNA Barcode:
CAGCTTTATGGCGTTGGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NRROS (375387)
Gene Description:
negative regulator of reactive oxygen species
Transcript:
RefSeq NM_198565.1 (NON-CURRENT)
Match location:
Position 1679 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTTTATGGCGTTGGACTT
Hairpin Sequence:
5'-CCGG-CAGCTTTATGGCGTTGGACTT-CTCGAG-AAGTCCAACGCCATAAAGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTTTATGGCGTTGGACTTCTCGAGAAGTCCAACGCCATAAAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTTTATGGCGTTGGACTTCTCGAGAAGTCCAACGCCATAAAGCTG-3'

Other clones with same target sequence:

(none)