Construct: shRNA TRCN0000131048

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007341.1-407s1c1
DNA Barcode:
CCAGATCTTCAATGAGGAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BGR (6450)
Gene Description:
SH3 domain binding glutamate rich protein
Transcript:
RefSeq NM_007341.1 (NON-CURRENT)
Match location:
Position 407 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131048)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATCTTCAATGAGGAGCA
Hairpin Sequence:
5'-CCGG-CCAGATCTTCAATGAGGAGCA-CTCGAG-TGCTCCTCATTGAAGATCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATCTTCAATGAGGAGCACTCGAGTGCTCCTCATTGAAGATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATCTTCAATGAGGAGCACTCGAGTGCTCCTCATTGAAGATCTGG-3'

Other clones with same target sequence:

(none)