Construct: shRNA TRCN0000131072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_379802.1-1054s1c1
DNA Barcode:
CATGGAGAGTGTACCTGTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPY19L1P1 (89231)
Gene Description:
dpy-19-like 1 pseudogene 1 (C. elegans)
Transcript:
RefSeq XM_379802.1 (NON-CURRENT)
Match location:
Position 1054 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGAGAGTGTACCTGTGTA
Hairpin Sequence:
5'-CCGG-CATGGAGAGTGTACCTGTGTA-CTCGAG-TACACAGGTACACTCTCCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGAGAGTGTACCTGTGTACTCGAGTACACAGGTACACTCTCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGAGAGTGTACCTGTGTACTCGAGTACACAGGTACACTCTCCATG-3'

Other clones with same target sequence:

(none)