Construct: shRNA TRCN0000131077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178011.2-2765s1c1
DNA Barcode:
GCCCAGAAACCTTGACTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRTM3 (347731)
Gene Description:
leucine rich repeat transmembrane neuronal 3
Transcript:
RefSeq NM_178011.2 (NON-CURRENT)
Match location:
Position 2765 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGAAACCTTGACTCTAA
Hairpin Sequence:
5'-CCGG-GCCCAGAAACCTTGACTCTAA-CTCGAG-TTAGAGTCAAGGTTTCTGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGAAACCTTGACTCTAACTCGAGTTAGAGTCAAGGTTTCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGAAACCTTGACTCTAACTCGAGTTAGAGTCAAGGTTTCTGGGC-3'

Other clones with same target sequence:

(none)