Construct: shRNA TRCN0000131115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174908.2-1728s1c1
DNA Barcode:
GCCACCTCTATGCTGCATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC50 (152137)
Gene Description:
coiled-coil domain containing 50
Transcript:
RefSeq NM_174908.2 (NON-CURRENT)
Match location:
Position 1728 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTCTATGCTGCATATA
Hairpin Sequence:
5'-CCGG-GCCACCTCTATGCTGCATATA-CTCGAG-TATATGCAGCATAGAGGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTCTATGCTGCATATACTCGAGTATATGCAGCATAGAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTCTATGCTGCATATACTCGAGTATATGCAGCATAGAGGTGGC-3'

Other clones with same target sequence:

(none)