Construct: shRNA TRCN0000131116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013241.1-3529s1c1
DNA Barcode:
GAAATCTATCTGGACCCTGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FHOD1 (29109)
Gene Description:
formin homology 2 domain containing 1
Transcript:
RefSeq NM_013241.1 (NON-CURRENT)
Match location:
Position 3529 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATCTATCTGGACCCTGGA
Hairpin Sequence:
5'-CCGG-GAAATCTATCTGGACCCTGGA-CTCGAG-TCCAGGGTCCAGATAGATTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATCTATCTGGACCCTGGACTCGAGTCCAGGGTCCAGATAGATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATCTATCTGGACCCTGGACTCGAGTCCAGGGTCCAGATAGATTTC-3'

Other clones with same target sequence:

(none)